MiR-143-5p inhibits proliferation, invasion, and epithelial to mesenchymal transition of colorectal cancer cells by downregulation of HMGA2


 Purpose: To investigate the regulatory effect and molecular mechanism of miR-143-5p in colorectal cancer (CRC) progression.
 Methods: Expression CRC cell lines SW620 HCT116 was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Stable overexpression mediated lentivirus. The effects on proliferation, migration, invasion, epithelial- mesenchymal transition (EMT) cells were assessed colony formation assay, CCK-8, Transwell wound healing western blot. Target prediction performed for miR-143-5p, a dual luciferase assay used to verify targeting relationship.
 Results: Compared transfected with negative controls, migration EMT inhibited miR-143-5p-overexpressing cells. HMGA2 (high- mobility Group AT-Hook 2), target gene repressed miR-143-5p. Rescue experiments confirmed that upregulation due mIR-143-5p reversed inhibition invasion EMT.
 Conclusion: MiR-143-5p inhibits malignant progression regulating expression is expected provide new therapeutic approaches clinical treatment CRC.